Synaptoporin, a novel putative channel protein of synaptic vesicles

Neuron. 1990 Oct;5(4):453-62. doi: 10.1016/0896-6273(90)90084-s.


By homology screening of a rat brain library, we have isolated cDNAs that encode a novel member of the synaptophysin/connexin channel protein superfamily. The deduced protein, named synaptoporin, displays 58% amino acid identity to synaptophysin, with highly conserved transmembrane segments, but a divergent cytoplasmic tail. Northern blot analysis and PCR amplification of RNA from different rat tissues indicate expression of synaptoporin transcripts in the CNS. Antibodies against a synthetic peptide or a fusion construct encompassing the cytoplasmic tail region of synaptoporin detect a polypeptide of 37 kd that copurifies with small synaptic vesicles. Our data suggest the existence of a family of vesicular channel proteins whose members may be differently distributed among synaptic vesicle subpopulations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA / isolation & purification
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Nerve Tissue Proteins / metabolism*
  • RNA, Messenger / metabolism
  • Rats
  • Synaptic Vesicles / metabolism*
  • Synaptophysin
  • Tissue Distribution


  • Membrane Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Synaptophysin
  • Synpr protein, rat
  • DNA