Natural recovery from antiglomerular basement membrane glomerulonephritis is associated with glomeruli-infiltrating CD8α+CD11c+MHC class II+ cells

Am J Nephrol. 2011;34(6):519-28. doi: 10.1159/000333004. Epub 2011 Nov 7.


Background/aims: In an antiglomerular basement membrane glomerulonephritis (GN) model, GN-resistant Lewis (LEW) rats naturally recover from early glomerular inflammation (days 21-23). We have previously identified a glomeruli-infiltrating CD8α(+)CD11(high)MHC II(+) cell (GIL CD8α(+) cell) in GN-prone Wistar Kyoto (WKY) rats, which terminates glomerular inflammation through inducing T cell apoptosis prior to glomerular fibrosis at days 35-40. We investigated if GIL CD8α(+) cells were also associated with the recovery in LEW rats.

Methods: GIL CD8α(+) cells in LEW rats were characterized; their infiltration was observed in connection with T cell apoptosis in glomeruli.

Results: An influx of GIL CD8α(+) cells into inflamed glomeruli was confirmed in the immunized LEW rats at days 17-22, which was much earlier than days 28-35 in WKY rats. Notably, LEW rats had a GIL CD8α(+)CD11(high) subpopulation after day 17, while WKY rats lacked this population until after day 30. Analyses further revealed a large number of clustered apoptotic CD4(+) or CD3(+) T cells in the glomeruli during recovery (day 23) in LEW rats, as compared to day 35 (transition to fibrosis) in WKY rats. Thus, infiltration of GIL CD8α(+) cells coincided with decline of glomerular inflammation and T cell apoptosis during recovery in LEW rats. Isolated GIL CD8α(+) cells were able to infiltrate glomeruli in both WKY and LEW rats at day 20.

Conclusion: Our data revealed a strong association between GIL CD8a+ cells and recovery from early glomerular inflammation. It raises a possibility of involvement of GIL CD8a+ cells in the recovery.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Apoptosis
  • Autoantibodies / immunology*
  • CD11c Antigen / biosynthesis
  • CD8 Antigens / biosynthesis
  • Female
  • Flow Cytometry / methods
  • Glomerulonephritis / immunology*
  • Histocompatibility Antigens Class II / metabolism*
  • Inflammation
  • Kidney Glomerulus / immunology*
  • Kidney Glomerulus / metabolism
  • Microscopy, Confocal / methods
  • Rats
  • Rats, Inbred Lew
  • Rats, Inbred WKY
  • T-Lymphocytes / cytology
  • Time Factors


  • Autoantibodies
  • CD11c Antigen
  • CD8 Antigens
  • CD8alpha antigen
  • Histocompatibility Antigens Class II
  • antiglomerular basement membrane antibody