Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes

J Photochem Photobiol B. 2012 Jan 5:106:24-33. doi: 10.1016/j.jphotobiol.2011.09.006. Epub 2011 Oct 20.

Abstract

Hydrogen-rich electrolyzed warm water (HW) was prepared at 41°C and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation-reduction potential (ORP) of -741 mV in contrast to below 0.01 ppm and+184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used to examine effects of HW against UVA-ray irradiation. Type-I collagen was synthesized 1.85- to 2.03-fold more abundantly by HW application for 3-5 days than RW in OUMS-36 fibroblasts, and localized preferentially around the nuclei as shown by immunostain. HW application significantly prevented cell death and DNA damages such as nuclear condensation and fragmentation in UVA-irradiated HaCaT keratinocytes as estimated by WST-1 and Hoechst 33342 assays. HW significantly suppressed UVA-induced generation of intracellular superoxide anion radicals in both the cell lines according to NBT assay. Wrinkle repression was clinically assessed using a HW-bathing. Six Japanese subjects were enrolled in a trial of HW-bathing (DH, 0.2-0.4 ppm) every day for 3 months. HW-bathing significantly improved wrinkle in four subjects on the back of neck on 90th day as compared to 0 day. Thus, HW may serve as daily skin care to repress UVA-induced skin damages by ROS-scavenging and promotion of type-I collagen synthesis in dermis.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Apoptosis
  • Cell Line
  • Collagen Type I / metabolism*
  • DNA Damage
  • Dermis / metabolism
  • Female
  • Fibroblasts / drug effects*
  • Fibroblasts / radiation effects
  • Humans
  • Hydrogen / chemistry
  • Keratinocytes / drug effects*
  • Keratinocytes / radiation effects
  • Male
  • Middle Aged
  • Oxidative Stress
  • Reactive Oxygen Species / metabolism
  • Skin Aging / drug effects*
  • Skin Aging / pathology
  • Skin Aging / radiation effects
  • Superoxides / metabolism
  • Temperature
  • Ultraviolet Rays*
  • Water / pharmacology*

Substances

  • Collagen Type I
  • Reactive Oxygen Species
  • Water
  • Superoxides
  • Hydrogen