Preparation of encapsulated alliinase in alginate microparticles

Biotechnol Lett. 2012 Mar;34(3):515-8. doi: 10.1007/s10529-011-0791-5. Epub 2011 Nov 10.

Abstract

Alliinase (alkylsulphenate lyase, EC 4.4.1.4), which catalyses the production of allicin, was immobilized in alginate microparticles. Addition of pyridoxal 5'-phosphate to the microparticles enhanced alliinase activity. Encapsulated alliinase were significantly higher (30 and 22%, respectively) than those of non-encapsulated alliinase at 60°C and at pH 2. Therefore, microencapsulation of alliinase with alginate can offer an effective way of sustaining enzyme activity during oral administration and passage through the stomach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry*
  • Capsules / chemistry*
  • Carbon-Sulfur Lyases / chemistry
  • Carbon-Sulfur Lyases / metabolism*
  • Disulfides
  • Enzyme Activators / metabolism
  • Enzyme Stability
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism*
  • Glucuronic Acid / chemistry
  • Hexuronic Acids / chemistry
  • Hydrogen-Ion Concentration
  • Pyridoxal / analogs & derivatives
  • Pyridoxal / metabolism
  • Sulfinic Acids / metabolism
  • Temperature

Substances

  • Alginates
  • Capsules
  • Disulfides
  • Enzyme Activators
  • Enzymes, Immobilized
  • Hexuronic Acids
  • Sulfinic Acids
  • allicin
  • Pyridoxal
  • pyridoxal 5'-sulfate
  • Glucuronic Acid
  • Carbon-Sulfur Lyases
  • alliin lyase