Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF

J Biol Chem. 2012 Jan 2;287(1):429-437. doi: 10.1074/jbc.M111.271163. Epub 2011 Nov 9.


In epigenetic signaling pathways, histone tails are heavily modified, resulting in the recruitment of effector molecules that can influence transcription. One such molecule, plant homeodomain finger protein 20 (PHF20), uses a Tudor domain to read dimethyl lysine residues and is a known component of the MOF (male absent on the first) histone acetyltransferase protein complex, suggesting it plays a role in the cross-talk between lysine methylation and histone acetylation. We sought to investigate the biological role of PHF20 by generating a knockout mouse. Without PHF20, mice die shortly after birth and display a wide variety of phenotypes within the skeletal and hematopoietic systems. Mechanistically, PHF20 is not required for maintaining the global H4K16 acetylation levels or locus specific histone acetylation but instead works downstream in transcriptional regulation of MOF target genes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA-Binding Proteins
  • Female
  • Gene Expression Regulation / genetics*
  • Gene Knockout Techniques
  • Histone Acetyltransferases / metabolism*
  • Histones / chemistry
  • Histones / metabolism
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism
  • Lysine / metabolism*
  • Male
  • Mice
  • Transcription Factors
  • Transcription, Genetic / genetics


  • DNA-Binding Proteins
  • Histones
  • Homeodomain Proteins
  • PHF20 protein, mouse
  • Transcription Factors
  • Histone Acetyltransferases
  • Kat8 protein, mouse
  • Lysine

Associated data

  • GEO/GSE29306