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, 6 (11), e27229

Protozoan Parasite Toxoplasma Gondii Manipulates Mate Choice in Rats by Enhancing Attractiveness of Males

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Protozoan Parasite Toxoplasma Gondii Manipulates Mate Choice in Rats by Enhancing Attractiveness of Males

Shantala Arundathi Hari Dass et al. PLoS One.

Abstract

Females in various species typically avoid males infected with parasites, while parasite-free males advertise their status through conspicuous phenotypic traits. This process selects for heritable resistance and reduces direct exposure of the female to parasites. Coevolving parasites are likely to attempt to circumvent this obstacle. In this paper, we demonstrate a case of parasitic manipulation of host mate choice. We report that Toxoplasma gondii, a sexually transmitted infection of brown rats, enhances sexual attractiveness of infected males. Thus under some evolutionary niches, parasites can indeed manipulate host sexual signaling to their own advantage.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Toxoplasma gondii is transmitted through intercourse.
Toxoplasma gondii cysts were observed in epididymis of infected males (panel A), in vaginal lavage of naïve females mated with infected males (panel B) and in brain of pups derived from these mating (panel C). Scale bar = 10 µm. Parasites are stained red (anti-GFP antibody couples with fluorogenic detection using Cy3), cyst wall green (dolichos biflorus agglutinin coupled with fluorescein) and sperm nuclei in blue (DAPI).
Figure 2
Figure 2. Uninfected females preferred infected males.
Preference was quantified by comparing time spent by an estrus female in two opposing arms of an arena, each arm urine marked by either controls or males infected six weeks earlier (panel A; trial duration  = 1200 s). Ordinate depicts time spent in infected bisect divided by control bisect (ratio >5 assigned arbitrary value of 5). Each dot represents raw data from one female. Box plots depict median, 25th percentile and 75th percentile. Preference of females for males in each of the 12 unique pair was calculated by taking median of all females tested for that particular male (panel B; ordinate and abscissa depicts time spent in infected and control bisect, respectively). Mean and SEM of data used in scatter-plot are depicted in grey color. These results were confirmed using a different rat strain in a geographically distinct location (panel C; Long-Evans rats in SU, USA; in place of Wistar rats at NTU, Singapore). **, p<0.001; Chi2 test. In a competitive paced mating, females chose to participate in more mounts and intromissions with infected males (panel D; ordinate and abscissa depicts number of intromissions by infected and control males, respectively). *, p<0.01; one-sample t-test against chance, i.e. 1. Intromission data exhibited one outlier that has been removed from the graph. Mean and SEM are depicted in grey color.

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References

    1. Morran LT, Schmidt OG, Gelarden IA, Parrish RC, 2nd, Lively CM. Running with the Red Queen: host-parasite coevolution selects for biparental sex. Science. 2011;333:216–218. - PMC - PubMed
    1. Dawson RD, Bortolotti GR. Carotenoid-dependent coloration of male American kestrels predicts ability to reduce parasitic infections. Naturwissenschaften. 2006;93:597–602. - PubMed
    1. Deaton R. Effects of a parasitic nematode on male mate choice in a livebearing fish with a coercive mating system (western mosquitofish, Gambusia affinis). Behav Processes. 2009;80:1–6. - PubMed
    1. Ehman KD, Scott ME. Female mice mate preferentially with non-parasitized males. Parasitology. 2002;125:461–466. - PubMed
    1. Houde AE, Torio AJ. Effects of parasitic infection on male color pattern and female choice in guppies. Behavioral Ecology. 1992;3:346–351.

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