Abstract
We describe here the isolation of 8 beta-lactamase-producing multidrug-resistant Enterococcus faecium isolates in 2010. All strains showed diverse pulsed-field gel electrophoresis (PFGE) profiles, all belonging to the same clonal complex, CC17. By PCR and hybridization experiments, the entire blaZ-blaI-blaR1 operon was found. The beta-lactamase activity was demonstrated at a high inoculum and in the presence of methicillin after overnight incubation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Cluster Analysis
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DNA, Bacterial / genetics
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Drug Resistance, Multiple, Bacterial
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Electrophoresis, Gel, Pulsed-Field
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Enterococcus faecium / classification*
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Enterococcus faecium / drug effects
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Enterococcus faecium / enzymology*
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Enterococcus faecium / isolation & purification
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Genotype
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Gram-Positive Bacterial Infections / epidemiology*
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Gram-Positive Bacterial Infections / microbiology*
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Humans
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Methicillin / pharmacology
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Molecular Typing
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Nucleic Acid Hybridization
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Polymerase Chain Reaction
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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beta-Lactamases
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Methicillin