Polyclonal diffusion of beta-lactamase-producing Enterococcus faecium

J Clin Microbiol. 2012 Jan;50(1):169-72. doi: 10.1128/JCM.05640-11. Epub 2011 Nov 9.

Abstract

We describe here the isolation of 8 beta-lactamase-producing multidrug-resistant Enterococcus faecium isolates in 2010. All strains showed diverse pulsed-field gel electrophoresis (PFGE) profiles, all belonging to the same clonal complex, CC17. By PCR and hybridization experiments, the entire blaZ-blaI-blaR1 operon was found. The beta-lactamase activity was demonstrated at a high inoculum and in the presence of methicillin after overnight incubation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Cluster Analysis
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial
  • Electrophoresis, Gel, Pulsed-Field
  • Enterococcus faecium / classification*
  • Enterococcus faecium / drug effects
  • Enterococcus faecium / enzymology*
  • Enterococcus faecium / isolation & purification
  • Genotype
  • Gram-Positive Bacterial Infections / epidemiology*
  • Gram-Positive Bacterial Infections / microbiology*
  • Humans
  • Methicillin / pharmacology
  • Molecular Typing
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • beta-Lactamases
  • Methicillin