Resonance Raman quantification of the redox state of cytochromes b and c in-vivo and in-vitro

J Biophotonics. 2012 Jan;5(1):20-4. doi: 10.1002/jbio.201100087. Epub 2011 Nov 11.

Abstract

We observe the redox state changes with respiration of cytochromes b and c in mitochondria in a living Saccharomyces cerevisiae cell as well as in isolated mitochondria with the very use of Raman microspectroscopy. The possibility of monitoring the respiration activity of mitochondria in vivo and in vitro by Raman microspectroscopic quantification of the cytochrome redox states is suggested. It will lead to a new means to assess mitochondrial respiration activity in vivo and in vitro without using any labelling or genetic manipulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Respiration
  • Cytochrome c Group / metabolism*
  • Cytochromes b / metabolism*
  • Mitochondria / enzymology*
  • Oxidation-Reduction
  • Oxygen Consumption
  • Saccharomyces cerevisiae / metabolism*
  • Spectrum Analysis, Raman / methods*

Substances

  • Cytochrome c Group
  • Cytochromes b