Inhibitory constituents of Nardostachys chinensis on nitric oxide production in RAW 264.7 macrophages

Bioorg Med Chem Lett. 2012 Jan 1;22(1):706-8. doi: 10.1016/j.bmcl.2011.10.043. Epub 2011 Oct 18.

Abstract

The activity-guided fractionation of the MeOH extract of the rhizomes and roots of Nardostachys chinensis led to the isolation of two new sesquiterpenoids, narchinol B (8) and narchinol C (9), along with 10 known compounds, ursolic acid (1), nardosinone (2), pinoresinol (3), desoxo-narchinol A (4), kanshone B (5), epoxyconiferyl alcohol (6), debilon (7), 4α,5-dimethyl-1,3-dioxo-1,2,3,4,4α,5,6,7-octahydronaphthalene (10), p-coumaric acid (11), and isoferulic acid (12). Their structures were determined using spectroscopic techniques, which included 1D- and 2D-NMR. Among the isolates, compounds 2, 4, 5, 8 and 9 showed inhibitory activity against LPS-induced NO production with IC(50) values of 4.6-21.6 μM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drug Design
  • Inhibitory Concentration 50
  • Lipopolysaccharides / chemistry
  • Macrophages / cytology*
  • Magnetic Resonance Spectroscopy / methods
  • Methanol / chemistry
  • Mice
  • Models, Chemical
  • Nardostachys / metabolism*
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / chemistry
  • Nitric Oxide / metabolism*
  • Plant Extracts / pharmacology*
  • Plant Roots / metabolism
  • Rhizome / chemistry
  • Spectrophotometry / methods

Substances

  • Lipopolysaccharides
  • Plant Extracts
  • Nitric Oxide
  • Methanol