Abstract
Orthogonal amino acid reporters allow the selective labeling of different cell types in heterogeneous populations through the expression of engineered aminoacyl tRNA synthetases. Here, we demonstrate that para-ethynylphenylalanine (PEP) can be used as an orthogonal amino acid reporter for efficient selective labeling of an intracellular bacterial pathogen during infection.
This journal is © The Royal Society of Chemistry 2012
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alanine / analogs & derivatives*
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Alanine / chemistry
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Alanine / metabolism
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HeLa Cells
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Humans
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Isotope Labeling / methods*
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Methionine-tRNA Ligase / chemistry*
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Methionine-tRNA Ligase / genetics
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Methionine-tRNA Ligase / metabolism
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Microscopy, Fluorescence
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Proteome
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Receptors, Amino Acid / chemistry*
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Receptors, Amino Acid / metabolism
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Salmonella typhimurium / enzymology*
Substances
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Proteome
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Receptors, Amino Acid
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p-ethynylphenylalanine
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Methionine-tRNA Ligase
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Alanine