Abstract
FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined global RNA targets for all human FET proteins and two ALS-causing human FUS mutants. FET members showed similar binding profiles, whereas FUS mutants showed a drastically altered binding pattern, consistent with changes in subcellular localization.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyotrophic Lateral Sclerosis / genetics
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Base Sequence
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Binding Sites
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Calmodulin-Binding Proteins / chemistry
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Calmodulin-Binding Proteins / metabolism*
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HEK293 Cells
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Humans
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Mutation
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RNA, Messenger / chemistry
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RNA, Messenger / metabolism*
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RNA-Binding Protein EWS
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RNA-Binding Protein FUS / chemistry
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RNA-Binding Protein FUS / metabolism*
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / metabolism*
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Sequence Alignment
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Sequence Analysis, DNA
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TATA-Binding Protein Associated Factors / chemistry
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TATA-Binding Protein Associated Factors / metabolism*
Substances
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Calmodulin-Binding Proteins
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EWSR1 protein, human
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RNA, Messenger
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RNA-Binding Protein EWS
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RNA-Binding Protein FUS
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RNA-Binding Proteins
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TAF15 protein, human
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TATA-Binding Protein Associated Factors