Cytokinesis Remnants Define First Neuronal Asymmetry in Vivo

Nat Neurosci. 2011 Nov 13;14(12):1525-33. doi: 10.1038/nn.2976.

Abstract

Polarization of a neuron begins with the appearance of the first neurite, thus defining the ultimate growth axis. Unlike late occurring polarity events (such as axonal growth), very little is known about this fundamental process. We show here that, in Drosophila melanogaster neurons in vivo, the first membrane deformation occurred 3.6 min after precursor division. Clustering of adhesion complex components (Bazooka (Par-3), cadherin-catenin) marked this place by 2.8 min after division; the upstream phosphatidylinositol 4,5-bisphosphate, by 0.7 min after division; and the furrow components RhoA and Aurora kinase, from the time of cytokinesis. Local DE-cadherin inactivation prevented sprout formation, whereas perturbation of division orientation did not alter polarization from the cytokinesis pole. This is, to our knowledge, the first molecular study of initial neuronal polarization in vivo. The mechanisms of polarization seem to be defined at the precursor stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Cadherins / metabolism
  • Cell Adhesion Molecules / metabolism
  • Cell Cycle / genetics
  • Cell Differentiation
  • Cell Polarity / genetics
  • Cell Polarity / physiology*
  • Centrioles / physiology
  • Cytokinesis / genetics
  • Cytokinesis / physiology*
  • Drosophila
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism
  • Embryonic Development*
  • Gene Expression Regulation, Developmental / genetics
  • Gene Expression Regulation, Developmental / physiology*
  • Horseradish Peroxidase / metabolism
  • Imaging, Three-Dimensional
  • Luminescent Proteins / genetics
  • Microscopy, Confocal
  • Mutation / genetics
  • Phosphatidylinositol 4,5-Diphosphate / metabolism
  • Pupa
  • Sense Organs / cytology
  • Sense Organs / embryology
  • Sensory Receptor Cells / cytology
  • Sensory Receptor Cells / metabolism*
  • Time Factors
  • alpha Catenin / metabolism

Substances

  • Cadherins
  • Cell Adhesion Molecules
  • Drosophila Proteins
  • Luminescent Proteins
  • Phosphatidylinositol 4,5-Diphosphate
  • alpha Catenin
  • alpha-Cat protein, Drosophila
  • Horseradish Peroxidase