Abstract
We have recently identified seven mutations in commonly used stocks of the sequenced Escherichia coli strain MG1655 which do not appear in the reference sequence. The mutations are likely to cause loss of function of the glpR and crl genes, which may have serious implications for physiological experiments using the affected strains.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Base Sequence
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DNA-Directed RNA Polymerases / metabolism
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Escherichia coli K12 / classification*
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Escherichia coli K12 / genetics*
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Escherichia coli K12 / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Gene Expression Profiling
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Gene Expression Regulation, Bacterial / physiology
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Genetic Variation*
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Glycerophosphates / metabolism
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Mutation
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Sigma Factor / metabolism
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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GlpR protein, E coli
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Glycerophosphates
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Repressor Proteins
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Sigma Factor
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Crl protein, Bacteria
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alpha-glycerophosphoric acid
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DNA-Directed RNA Polymerases