Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium

Cell. 1990 Oct 5;63(1):195-201. doi: 10.1016/0092-8674(90)90300-4.


We have identified a novel growth factor, stem cell factor (SCF), for primitive hematopoietic progenitors based on its activity on bone marrow cells derived from mice treated with 5-fluorouracil. The protein was isolated from the medium conditioned by Buffalo rat liver cells. It is heavily glycosylated, with both N-linked and O-linked carbohydrate. Amino acid sequence following removal of N-terminal pyroglutamate is presented. The protein has potent synergistic activities in semisolid bone marrow cultures in conjunction with colony-stimulating factors. It is also a growth factor for mast cells. In two companion papers, we present the sequences of partial SCF cDNAs, identify SCF as a c-kit ligand, and map the SCF gene to the Sl locus of the mouse.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bone Marrow Cells
  • Cell Division / drug effects
  • Cells, Cultured
  • Chromatography, Affinity
  • Chromatography, Ion Exchange
  • Culture Media
  • Fluorouracil / pharmacology
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Hematopoietic Cell Growth Factors / biosynthesis
  • Hematopoietic Cell Growth Factors / isolation & purification*
  • Hematopoietic Cell Growth Factors / pharmacology
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / drug effects
  • Liver / physiology*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Rats
  • Rats, Inbred BUF


  • Culture Media
  • Hematopoietic Cell Growth Factors
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Fluorouracil