CD83(+) dendritic cells and Foxp3(+) regulatory T cells in primary lesions and regional lymph nodes are inversely correlated with prognosis of gastric cancer

Gastric Cancer. 2012 Apr;15(2):144-53. doi: 10.1007/s10120-011-0090-9. Epub 2011 Nov 15.

Abstract

Background: Dendritic cells (DCs) are potent antigen-presenting cells that are central to the regulation, maturation, and maintenance of the cellular immune response against cancer. In contrast, CD4(+)CD25(+) regulatory T cells (Tregs) play a central role in self-tolerance and suppress antitumor immunity. In this study, we investigated the clinical significance of mature CD83(+) DCs and Foxp3(+) Tregs in the primary tumor and regional lymph nodes from the viewpoint of the two opposing players in the immune responses.

Methods: We investigated, immunohistochemically, the density of CD83(+) DCs and Foxp3(+) Tregs in primary lesions of gastric cancer (n = 123), as well as in regional lymph nodes with (n = 40) or without metastasis (n = 40).

Results: Decreased density of CD83(+) DCs and increased density of Foxp3(+) Tregs were observed in the primary tumor and metastatic lymph nodes. Density was significantly correlated with certain clinicopathological features. Poor prognosis was observed in patients with a low density of CD83(+) DCs and a high density of Foxp3(+) Tregs in primary lesions. For patients with metastatic lymph nodes, the density of CD83(+) DCs in negative lymph nodes was found to be an independent prognostic factor by multivariate analysis.

Conclusion: The density of CD83(+) DCs and Foxp3(+) Tregs was inversely correlated with tumor progression and reflected the prognosis of gastric cancer.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigen-Presenting Cells / immunology*
  • Antigens, CD / analysis*
  • Dendritic Cells / immunology*
  • Female
  • Forkhead Transcription Factors / analysis*
  • Humans
  • Immune Tolerance
  • Immunohistochemistry
  • Kaplan-Meier Estimate
  • Lymph Nodes / immunology*
  • Lymph Nodes / pathology
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Prognosis
  • Signaling Lymphocytic Activation Molecule Family
  • Stomach Neoplasms / immunology*
  • Stomach Neoplasms / mortality
  • Stomach Neoplasms / pathology
  • Survival Rate
  • T-Lymphocytes, Regulatory / immunology*
  • Young Adult

Substances

  • Antigens, CD
  • CD84 protein, human
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Signaling Lymphocytic Activation Molecule Family