Abstract
Broad-host-range plasmid pRIO-5, harboring the extended-spectrum β-lactamase bla(BES-1) gene in Serratia marcescens, was fully sequenced. Analysis of the 12,957-bp sequence of this IncP6-type plasmid revealed that the bla(BES-1) gene was associated with two copies of the insertion sequence IS26. The promoter responsible for the bla(BES-1) expression was hybrid, made of a -35 box located inside the inverted repeat of IS26 and a -10 box inside a remnant of an insertion sequence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter baumannii / drug effects*
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Acinetobacter baumannii / genetics
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Anti-Bacterial Agents / pharmacology
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DNA Transposable Elements / genetics
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Escherichia coli / drug effects
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Escherichia coli / genetics*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Plasmids / chemistry
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Plasmids / genetics*
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Sequence Analysis, DNA*
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Serratia marcescens / drug effects
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Serratia marcescens / genetics*
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
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beta-Lactams / pharmacology
Substances
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Anti-Bacterial Agents
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DNA Transposable Elements
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beta-Lactams
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beta-lactamase BES-1
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beta-Lactamases