In vitro activity of Paris polyphylla smith against enterovirus 71 and coxsackievirus B3 and its immune modulation

Am J Chin Med. 2011;39(6):1219-34. doi: 10.1142/S0192415X11009512.

Abstract

Enterovirus 71 (EV71) and coxsackievirus B3 (CVB3) have resulted in severe pathogenesis caused by the host's immune response, including the cytokine cascade. Paris polyphylla Smith is a folk medicinal plant in Asia traditionally prescribed for the reduction of pain and elimination of poisoning. In this study, we investigated the anti-EV71 and CVB3 activity of P. polyphylla Smith as well as its immune modulation. The IC(50) for the P. polyphylla Smith 95% ethanol extract against EV71 and CVB3 were 12.5-23% and 99-156% of that of ribavirin, a positive control. Prevention of viral infection, viral inactivation, and anti-viral replication effects against both EV71 and CVB3 were demonstrated by the extract, the anti-viral replication effect being dominant. The extract significantly increased IL-6 production in both EV71- and CVB3-infected cells. A high correlation was possibly demonstrated between the high amounts of IL-6 induction in the EV71 and CVB3-infected cells and the anti-viral replication activity of the extract. In conclusion, good anti-EV71 and CVB3 activity was observed in the P. polyphylla Smith 95% ethanol extract. The high amounts of IL-6 induction in the virus-infected cells played a key role in the anti-viral activity of the extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / pharmacology*
  • Cell Line
  • Coxsackievirus Infections / immunology
  • Coxsackievirus Infections / virology*
  • Cytokines / immunology
  • Enterovirus A, Human / drug effects*
  • Enterovirus A, Human / physiology
  • Enterovirus B, Human / drug effects*
  • Enterovirus B, Human / physiology
  • Humans
  • Immunologic Factors / pharmacology*
  • Liliaceae / chemistry*
  • Plant Extracts / pharmacology*

Substances

  • Antiviral Agents
  • Cytokines
  • Immunologic Factors
  • Plant Extracts