In situ DNA sequence mapping with surface-spread mouse pachytene chromosomes

Cytogenet Cell Genet. 1990;53(4):219-20. doi: 10.1159/000132935.

Abstract

Surface-spread pachytene chromosomes are several times the length of metaphase chromosomes and the decondensed chromatin loops are attached to a well-defined axis (Weith and Traut, 1980). This arrangement permits detailed DNA sequence localization by in situ hybridization. We show that two probes to low-frequency repeated sequences (20 to 50 copies) which locate the centromere proximal in the mouse X metaphase chromosome between bands A1 and A3 (Disteche et al., 1985) and which map 5.5 cM apart (Disteche et al., 1989), hybridize to two distinct chromatin regions 3 to 5 microns apart on a 25 microns long pachytene X chromosome core.

MeSH terms

  • Animals
  • Chromosome Mapping / methods*
  • DNA Probes
  • Meiosis*
  • Mice
  • Nucleic Acid Hybridization
  • Repetitive Sequences, Nucleic Acid
  • X Chromosome / ultrastructure

Substances

  • DNA Probes