Hydrogen-rich water attenuates experimental periodontitis in a rat model

J Clin Periodontol. 2011 Dec;38(12):1085-90. doi: 10.1111/j.1600-051X.2011.01801.x. Epub 2011 Oct 9.


Aim: Reactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen-rich water on a rat model of periodontitis.

Material & methods: A ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen-rich water.

Results: The rats with periodontitis which were treated with pure water showed a time-dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis-induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen-rich water intake inhibited an increase in serum ROS level and lowered expression of 8-hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen-activated protein kinases, were less activated in periodontal lesions from hydrogen-rich water-treated rats as compared with pure water-treated rats.

Conclusion: Consuming hydrogen-rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Free Radical Scavengers / therapeutic use*
  • Gingiva / metabolism
  • Hydrogen / chemistry
  • Hydrogen / therapeutic use*
  • Male
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology
  • Periodontitis / blood
  • Periodontitis / drug therapy
  • Periodontitis / prevention & control*
  • Proton Therapy
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species / blood
  • Single-Blind Method
  • Water / chemistry


  • Free Radical Scavengers
  • Reactive Oxygen Species
  • Water
  • Hydrogen