Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus

J Med Virol. 2012 Jan;84(1):87-95. doi: 10.1002/jmv.22228.

Abstract

Andes virus (ANDV) is responsible for the Hantavirus Pulmonary Syndrome cases in Argentina and neighboring countries, with moderate to high case-fatality rates. ANDV has some particular features, which make it unique among other members of the Hantavirus genus such as person-to-person transmission and causing a disease similar to Hantavirus Pulmonary Syndrome in the hamster as an animal model. The kinetics of replication in Vero E6 cells of an ANDV strain isolated in Argentina, called Andes/ARG, was studied. Cytopathic effect and the formation of clear plaques were observed and therefore Andes/ARG could be quantified by classic plaque assay. The Andes/ARG strain was found to be highly lethal in Syrian hamsters allowing experiments to demonstrate the protective potential of vaccines. A recombinant nucleocapsid protein of ANDV induced a long lasting antibody response and protective immunity against a homologous challenge, but to a lower extent against heterologous challenge by the Seoul virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Chlorocebus aethiops
  • Cricetinae
  • Cytopathogenic Effect, Viral
  • Disease Models, Animal
  • Hantavirus / immunology*
  • Hantavirus / pathogenicity
  • Hantavirus Infections / immunology
  • Hantavirus Infections / mortality
  • Hantavirus Infections / pathology
  • Hantavirus Infections / prevention & control*
  • Male
  • Mesocricetus
  • Seoul virus / immunology
  • Survival Analysis
  • Vero Cells
  • Viral Plaque Assay

Substances

  • Antibodies, Viral