The structure of the eukaryotic ribosome at 3.0 Å resolution

Science. 2011 Dec 16;334(6062):1524-9. doi: 10.1126/science.1212642. Epub 2011 Nov 17.


Ribosomes translate genetic information encoded by messenger RNA into proteins. Many aspects of translation and its regulation are specific to eukaryotes, whose ribosomes are much larger and intricate than their bacterial counterparts. We report the crystal structure of the 80S ribosome from the yeast Saccharomyces cerevisiae--including nearly all ribosomal RNA bases and protein side chains as well as an additional protein, Stm1--at a resolution of 3.0 angstroms. This atomic model reveals the architecture of eukaryote-specific elements and their interaction with the universally conserved core, and describes all eukaryote-specific bridges between the two ribosomal subunits. It forms the structural framework for the design and analysis of experiments that explore the eukaryotic translation apparatus and the evolutionary forces that shaped it.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • DNA-Binding Proteins / ultrastructure
  • Models, Molecular
  • RNA, Fungal / ultrastructure
  • RNA, Ribosomal / ultrastructure
  • Ribosomes / ultrastructure*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / ultrastructure*
  • Saccharomyces cerevisiae Proteins / ultrastructure


  • DNA-Binding Proteins
  • RNA, Fungal
  • RNA, Ribosomal
  • Saccharomyces cerevisiae Proteins
  • Stm1 protein, S cerevisiae

Associated data

  • PDB/3U5B
  • PDB/3U5C
  • PDB/3U5D
  • PDB/3U5E
  • PDB/3U5F
  • PDB/3U5G
  • PDB/3U5H
  • PDB/3U5I