[Application of the Duplex-Specific Nuclease for Fast Analysis of Single Nucleotide Polymorphisms and Detection of Target DNA in Complex PCR Products]

Bioorg Khim. Jul-Aug 2011;37(4):522-9. doi: 10.1134/s1068162011040121.
[Article in Russian]

Abstract

We have developed a simple method for fast analysis of single nucleotide polymorphisms and identification of target clones from cloned complex PCR products. The method utilizes Kamchatka crab duplex-specific nuclease and universal fluorescent probe and is alternative to laborious screening procedures using radioactive probes, restriction analysis followed by gel electrophoresis or expensive sequencing. The method efficacy was demonstrated in several model experiments.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Brachyura / enzymology*
  • Cell Cycle Proteins / analysis
  • Cloning, Molecular
  • DNA / chemistry
  • DNA / genetics*
  • DNA Mutational Analysis / methods*
  • Deoxyribonucleases / chemistry*
  • Humans
  • Nuclear Proteins / analysis
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide / genetics*
  • Receptors, Antigen, T-Cell / analysis
  • Receptors, Antigen, T-Cell / chemistry

Substances

  • Cell Cycle Proteins
  • DKC1 protein, human
  • Nuclear Proteins
  • Receptors, Antigen, T-Cell
  • DNA
  • Deoxyribonucleases