Dietary treatments enriched in olive and safflower oils regulate seric and placental matrix metalloproteinases in maternal diabetes

Placenta. 2012 Jan;33(1):8-16. doi: 10.1016/j.placenta.2011.10.015. Epub 2011 Nov 17.


Objectives: Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in placental development and function, although related to the pro-inflammatory environment when produced in excess. Previous studies have identified MMP-2 and MMP-9 overactivities in the placenta from diabetic rats. In this study, we aimed to determine whether diets supplemented with olive and safflower oil, enriched in natural PPAR ligands, are able to regulate MMP-2 and MMP-9 activities in the placenta and serum from diabetic rats.

Study design: Diabetes was induced in rat neonates by streptozotocin administration (90mg/kg s.c.). Control and diabetic rats were fed with 6% olive oil- or 6% safflower oil-supplemented diets from days 0.5-13.5 of gestation.

Main outcome measures: On day 13.5 of gestation, placentas and sera were isolated for further determination of matrix metalloproteinases (MMPs) 2 and 9 activities by zymography. Placental MMP-2 and MMP-9 protein concentration and immunolocalization were also determined.

Results: Sera from diabetic pregnant animals showed MMP-2 and MMP-9 overactivities when compared to controls. Serum MMP-9 activity was significantly decreased when the diabetic animals received the olive and safflower oil dietary treatments. Placentas from diabetic rats showed increased MMP-2 and MMP-9 activities and protein concentrations, and both were decreased when diabetic rats received the olive and safflower dietary treatments.

Conclusions: This study demonstrates that both olive and safflower oil-supplemented diets were able to prevent MMPs overactivities in the placenta from diabetic rats, and that these beneficial effects are reflected in rat sera.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
  • Biomarkers / blood
  • Enzyme Precursors / metabolism
  • Female
  • Ligands
  • Matrix Metalloproteinase 2 / blood
  • Matrix Metalloproteinase 2 / metabolism*
  • Matrix Metalloproteinase 9 / blood
  • Matrix Metalloproteinase 9 / metabolism*
  • Olive Oil
  • Peroxisome Proliferator-Activated Receptors / agonists
  • Placenta / immunology
  • Placenta / metabolism*
  • Placenta / pathology
  • Plant Oils / therapeutic use*
  • Pregnancy
  • Pregnancy Proteins / blood
  • Pregnancy Proteins / metabolism
  • Pregnancy in Diabetics / diet therapy*
  • Pregnancy in Diabetics / immunology
  • Pregnancy in Diabetics / metabolism
  • Pregnancy in Diabetics / pathology
  • Protein Transport
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Safflower Oil / therapeutic use*
  • Streptozocin


  • Anti-Inflammatory Agents, Non-Steroidal
  • Biomarkers
  • Enzyme Precursors
  • Ligands
  • Olive Oil
  • Peroxisome Proliferator-Activated Receptors
  • Plant Oils
  • Pregnancy Proteins
  • Streptozocin
  • Safflower Oil
  • pro-matrix metalloproteinase 9
  • Matrix Metalloproteinase 2
  • Mmp2 protein, rat
  • Matrix Metalloproteinase 9