l-Arabinose production from sugar beet arabinan by immobilized endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus in a packed-bed reactor

Yeong-Su Kim; Yu-Ri Lim; Deok-Kun Oh

doi:10.1016/j.jbiosc.2011.10.008

l-Arabinose production from sugar beet arabinan by immobilized endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus in a packed-bed reactor

J Biosci Bioeng. 2012 Feb;113(2):239-41. doi: 10.1016/j.jbiosc.2011.10.008. Epub 2011 Nov 17.

Authors

Yeong-Su Kim¹, Yu-Ri Lim, Deok-Kun Oh

Affiliation

¹ Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143–701, Republic of Korea.

PMID: 22099373
DOI: 10.1016/j.jbiosc.2011.10.008

Abstract

The immobilized endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus produced continuously an average of 16.5 gl(-1)l-arabinose from 20 gl(-1) sugar beet arabinan at pH 5.0 and 75°C for 216 h, with a productivity of 9.9 gl(-1)h(-1) and a conversion yield of 83%.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabinose / biosynthesis*
Beta vulgaris / chemistry*
Bioreactors
Enzymes, Immobilized / metabolism
Glycoside Hydrolases / metabolism*
Gram-Positive Bacteria / enzymology*
Hydrogen-Ion Concentration
Kinetics
Polysaccharides / metabolism*
Temperature

Substances

Enzymes, Immobilized
Polysaccharides
araban
Arabinose
Glycoside Hydrolases
exo-alpha-L-arabinanase
arabinan endo-1,5-alpha-L-arabinosidase