Modified gold surfaces by poly(amidoamine) dendrimers and fructose dehydrogenase for mediated fructose sensing

Talanta. 2011 Dec 15;87:67-73. doi: 10.1016/j.talanta.2011.09.042. Epub 2011 Sep 29.

Abstract

An electrochemical biosensor for detection of fructose in food samples was developed by immobilization of fructose dehydrogenase (FDH) on cysteamine and poly(amidoamine) dendrimers (PAMAM)-modified gold electrode surface. Electrochemical analysis was carried out by using hexacyanoferrate (HCF) as a mediator and the response time was 35s at +300 mV vs. Ag/AgCl. Moreover, some parameters such as pH, enzyme loading and type of PAMAM (Generations 2, 3 and 4) were investigated. Then, the FDH biosensor was calibrated for fructose in the concentration range of 0.25-5.0mM. To evaluate its utility, the FDH biosensor was applied for fructose analysis in real samples. Finally, obtained data were compared with those measured with HPLC as a reference method.

Publication types

  • Evaluation Study

MeSH terms

  • Biosensing Techniques / economics
  • Biosensing Techniques / methods*
  • Carbohydrate Dehydrogenases / chemistry
  • Carbohydrate Dehydrogenases / metabolism*
  • Dendrimers / chemistry*
  • Electrochemical Techniques / economics
  • Electrochemical Techniques / methods
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism*
  • Fructose / analysis*
  • Gluconobacter / enzymology*
  • Gold / chemistry*
  • Sensitivity and Specificity
  • Surface Properties

Substances

  • Dendrimers
  • Enzymes, Immobilized
  • PAMAM Starburst
  • Fructose
  • Gold
  • Carbohydrate Dehydrogenases
  • D-fructose 5-dehydrogenase