Preparation and investigation of bioactive transferrin-iron complexes formed with different synergistic anions

Protein J. 2012 Jan;31(1):27-34. doi: 10.1007/s10930-011-9370-9.

Abstract

Human serum transferrin has a potential for drug-delivery systems. Oxalate and aziridine-carboxylate was conjugated to serum transferrin in order to transport into the targeted cancer cells via transferrin-receptor mediated endocytosis. Capillary zone electrophoresis and capillary isoelectric focusing were used to analyze the effectiveness of complexation reactions. The electropherograms show the differences between iron-free- and iron-complexed molecular forms of human serum transferrin. The iron-complexed transferrin sample containing the different anions as synergistic complexing agents were characterized by different electrophoretic parameters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anions / chemistry*
  • Anions / metabolism
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / metabolism
  • Antineoplastic Agents / pharmacology*
  • Aziridines / chemistry
  • Aziridines / metabolism
  • Aziridines / pharmacology
  • Biological Transport
  • Cell Line, Tumor
  • Drug Delivery Systems
  • Electrophoresis, Capillary
  • Humans
  • Iron / chemistry*
  • Iron / metabolism
  • Isoelectric Focusing
  • Oxalates / chemistry
  • Oxalates / metabolism
  • Oxalates / pharmacology
  • Protein Binding
  • Transferrin / chemistry*
  • Transferrin / metabolism

Substances

  • Anions
  • Antineoplastic Agents
  • Aziridines
  • Oxalates
  • Transferrin
  • aziridine-2-carboxylic acid
  • Iron