Human Suv3 protein reveals unique features among SF2 helicases

Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):988-96. doi: 10.1107/S0907444911040248. Epub 2011 Oct 19.

Abstract

Suv3 is a helicase that is involved in efficient turnover and surveillance of RNA in eukaryotes. In vitro studies show that human Suv3 (hSuv3) in complex with human polynucleotide phosphorylase has RNA degradosome activity. The enzyme is mainly localized in mitochondria, but small fractions are found in cell nuclei. Here, two X-ray crystallographic structures of human Suv3 in complex with AMPPNP, a nonhydrolysable analog of ATP, and with a short five-nucleotide strand of RNA are presented at resolutions of 2.08 and 2.9 Å, respectively. The structure of the enzyme is very similar in the two complexes and consists of four domains. Two RecA-like domains form the tandem typical of all helicases from the SF2 superfamily which together with the C-terminal all-helical domain makes a ring structure through which the nucleotide strand threads. The mostly helical N-terminal domain is positioned externally with respect to the core of the enzyme. Most of the typical helicase motifs are present in hSuv3, but the protein shows certain unique characteristics, suggesting that Suv3 enzymes may constitute a separate subfamily of helicases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenylyl Imidodiphosphate / chemistry
  • Adenylyl Imidodiphosphate / metabolism
  • Amino Acid Motifs
  • Crystallization
  • DEAD-box RNA Helicases / chemistry*
  • DEAD-box RNA Helicases / metabolism
  • Endoribonucleases / metabolism*
  • Humans
  • In Vitro Techniques
  • Mitochondria / enzymology*
  • Multienzyme Complexes / metabolism*
  • Nuclear Proteins / chemistry*
  • Polyribonucleotide Nucleotidyltransferase / metabolism*
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • RNA / chemistry
  • RNA / metabolism
  • RNA Helicases / metabolism*
  • RNA Splicing*
  • RNA-Binding Proteins / chemistry*
  • Serine-Arginine Splicing Factors
  • Structure-Activity Relationship

Substances

  • Multienzyme Complexes
  • Nuclear Proteins
  • RNA-Binding Proteins
  • degradosome
  • Serine-Arginine Splicing Factors
  • Adenylyl Imidodiphosphate
  • RNA
  • Polyribonucleotide Nucleotidyltransferase
  • Endoribonucleases
  • SUPV3L1 protein, human
  • DEAD-box RNA Helicases
  • RNA Helicases

Associated data

  • PDB/3RC3
  • PDB/3RC8