Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli

Karl Skoog; Bill Söderström; Jerker Widengren; Gunnar von Heijne; Daniel O Daley

doi:10.1128/JB.06091-11

Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli

J Bacteriol. 2012 Feb;194(3):584-6. doi: 10.1128/JB.06091-11. Epub 2011 Nov 18.

Authors

Karl Skoog¹, Bill Söderström, Jerker Widengren, Gunnar von Heijne, Daniel O Daley

Affiliation

¹ Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.

Abstract

To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Division*
Cytoplasm / genetics
Cytoplasm / metabolism*
Escherichia coli / chemistry
Escherichia coli / cytology*
Escherichia coli / genetics
Escherichia coli / metabolism
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Fluorescence Recovery After Photobleaching
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Periplasm / genetics
Periplasm / metabolism*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism

Substances

Escherichia coli Proteins
Recombinant Fusion Proteins
Green Fluorescent Proteins