A Shared Structural Solution for Neutralizing Ebolaviruses

Nat Struct Mol Biol. 2011 Nov 20;18(12):1424-7. doi: 10.1038/nsmb.2150.

Abstract

Sudan virus (genus Ebolavirus) is lethal, yet no monoclonal antibody is known to neutralize it. We here describe antibody 16F6 that neutralizes Sudan virus and present its structure bound to the trimeric viral glycoprotein. Unexpectedly, the 16F6 epitope overlaps that of KZ52, the only other antibody against the GP(1,2) core to be visualized to date. Furthermore, both antibodies against this crucial epitope bridging GP1-GP2 neutralize at a post-internalization step--probably fusion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / physiology
  • Antibodies, Neutralizing / chemistry*
  • Antibodies, Neutralizing / physiology
  • Crystallography, X-Ray
  • Ebolavirus / immunology*
  • Epitopes / chemistry
  • HEK293 Cells
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Models, Immunological
  • Models, Molecular
  • Neutralization Tests
  • Protein Structure, Tertiary
  • Viral Fusion Proteins / chemistry
  • Viral Fusion Proteins / immunology*
  • Viral Fusion Proteins / physiology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Epitopes
  • Viral Fusion Proteins

Associated data

  • PDB/3S88