Exercise-induced oxidative stress: the effects of β-alanine supplementation in women

Amino Acids. 2012 Jul;43(1):77-90. doi: 10.1007/s00726-011-1158-x. Epub 2011 Nov 20.


The purpose of this study was to evaluate the effects of β-alanine supplementation on markers of oxidative stress. Twenty-four women (age: 21.7±2.1 years; VO2max: 2.6±0.3 l min(-1)) were randomly assigned, in a double-blind fashion, to a β-alanine (BA, 2×800 mg tablets, 3× daily; CarnoSyn®; n=13) or placebo (PL, 2×800 mg maltodextrin tablets, 3× daily; n=11) group. A graded oxygen consumption test (VO2max) was performed to evaluate VO2max, time to exhaustion, ventilatory threshold and establish peak velocity (PV). A 40-min treadmill run was used to induce oxidative stress. Total antioxidant capacity, superoxide dismutase, 8-isoprostane (8ISO) and reduced glutathione were measured. Heart rate and ratings of perceived exertion were recorded during the 40 min run. Separate three- [4×2×2; acute (base vs. IP vs. 2 vs. 4 h)×chronic (pre- vs. post-)×treatment (BA vs. PL)] and two- [2×2; time (pre-supplement vs. post-supplement)×treatment (BA vs. PL)] way ANOVAs were used for analyses. There was a significant increase in VO2max (p=0.009), independent of treatment, with no significant changes in TTE (p=0.074) or VT (p=0.344). Ratings of perceived exertion values were significantly improved from pre- to post-supplementation for the BA group only at 40 min (p=0.02). The ANOVA model demonstrated no significant treatment effects on oxidative stress. The chronic effects of BA supplementation demonstrated little antioxidant potential, in women, and little influence on aerobic performance assessments.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carnosine / metabolism
  • Dietary Supplements
  • Dinoprost / analogs & derivatives
  • Dinoprost / blood
  • Double-Blind Method
  • Exercise Test / drug effects
  • Female
  • Glutathione / blood
  • Heart Rate / drug effects
  • Humans
  • Oxidative Stress / drug effects*
  • Oxygen Consumption / drug effects*
  • Physical Endurance / drug effects*
  • Reactive Oxygen Species / metabolism
  • Running / physiology*
  • Superoxide Dismutase / blood
  • Young Adult
  • beta-Alanine / administration & dosage
  • beta-Alanine / pharmacology*


  • Reactive Oxygen Species
  • beta-Alanine
  • 8-epi-prostaglandin F2alpha
  • Carnosine
  • Dinoprost
  • Superoxide Dismutase
  • Glutathione