Activation of microbubbles by short-pulsed ultrasound enhances the cytotoxic effect of cis-diamminedichloroplatinum (II) in a canine thyroid adenocarcinoma cell line in vitro

Ultrasound Med Biol. 2012 Jan;38(1):109-18. doi: 10.1016/j.ultrasmedbio.2011.09.017. Epub 2011 Nov 21.

Abstract

Ultrasound targeted microbubble destruction has succeeded in delivering drugs and genes. This study was designed to explore characteristics of ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound. Canine thyroid adenocarcinoma cells were exposed to short-pulsed diagnostic ultrasound in the presence of cis-diamminedichloroplatinum (II) (cisplatin) and ultrasound contrast agent Sonazoid(®) microbubbles. The cytotoxic effect of cisplatin was enhanced by short-pulsed diagnostic ultrasound and microbubbles. Incubation time with microbubbles influenced the cytotoxic effect of cisplatin. However, exposure duration did not affect the cytotoxic effect of cisplatin. Therefore, short-pulsed diagnostic ultrasound may activate microbubbles near cells and deliver cisplatin into cells. In addition, activation of microbubbles may be concluded in a short time. Our results suggest that short exposure duration could be potentially sufficient to induce efficient drug delivery by ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / physiopathology*
  • Adenocarcinoma / therapy
  • Animals
  • Antineoplastic Agents / administration & dosage
  • Cell Line, Tumor
  • Cell Survival / radiation effects
  • Cisplatin / administration & dosage*
  • Contrast Media / radiation effects
  • Contrast Media / therapeutic use
  • Dogs
  • Drug Synergism
  • Ferric Compounds / radiation effects*
  • Ferric Compounds / therapeutic use*
  • High-Energy Shock Waves*
  • Iron / radiation effects*
  • Iron / therapeutic use*
  • Microbubbles
  • Oxides / radiation effects*
  • Oxides / therapeutic use*
  • Thyroid Neoplasms / physiopathology*
  • Thyroid Neoplasms / therapy
  • Treatment Outcome

Substances

  • Antineoplastic Agents
  • Contrast Media
  • Ferric Compounds
  • Oxides
  • Sonazoid
  • Iron
  • Cisplatin