Abstract
"Bulb" is a mobile and complex structure appearing in vacuolar membrane of plant cell. We recently reported new fluorescent marker lines for bulbs and bulb-less mutants. We tried multicolor visualization of vacuolar membrane to show distinct segregation of bulb-positive protein (γTIP or AtVAM3) and bulb-negative protein (AtRab75). Unexpectedly, GFP-AtRab75 resulted to localize in bulb under the condition of co-expression with TagRFP-AtVAM3. The signal intensities of GFP-AtRab75 and TagRFP-AtVAM3 were quantified and compared. The result indicates that TagRFP-AtVAM3 is concentrated in bulb than GFP-AtRab75.
© 2011 Landes Bioscience
MeSH terms
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Acyltransferases / genetics
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Acyltransferases / metabolism
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Cell Membrane / ultrastructure*
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Green Fluorescent Proteins / metabolism
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Plants, Genetically Modified / genetics
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Plants, Genetically Modified / metabolism
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Qa-SNARE Proteins / genetics
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Qa-SNARE Proteins / metabolism*
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Vacuoles / ultrastructure*
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rab GTP-Binding Proteins / genetics
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rab GTP-Binding Proteins / metabolism*
Substances
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Arabidopsis Proteins
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Qa-SNARE Proteins
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Vam3 protein, Arabidopsis
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Green Fluorescent Proteins
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Acyltransferases
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TIP1 protein, Arabidopsis
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RabG3b protein, Arabidopsis
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rab GTP-Binding Proteins