Qualitative Difference Between "Bulb" Membranes and Other Vacuolar Membranes

Plant Signal Behav. 2011 Dec;6(12):1914-7. doi: 10.4161/psb.6.12.18061.

Abstract

"Bulb" is a mobile and complex structure appearing in vacuolar membrane of plant cell. We recently reported new fluorescent marker lines for bulbs and bulb-less mutants. We tried multicolor visualization of vacuolar membrane to show distinct segregation of bulb-positive protein (γTIP or AtVAM3) and bulb-negative protein (AtRab75). Unexpectedly, GFP-AtRab75 resulted to localize in bulb under the condition of co-expression with TagRFP-AtVAM3. The signal intensities of GFP-AtRab75 and TagRFP-AtVAM3 were quantified and compared. The result indicates that TagRFP-AtVAM3 is concentrated in bulb than GFP-AtRab75.

MeSH terms

  • Acyltransferases / genetics
  • Acyltransferases / metabolism
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Membrane / ultrastructure*
  • Green Fluorescent Proteins / metabolism
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Qa-SNARE Proteins / genetics
  • Qa-SNARE Proteins / metabolism*
  • Vacuoles / ultrastructure*
  • rab GTP-Binding Proteins / genetics
  • rab GTP-Binding Proteins / metabolism*

Substances

  • Arabidopsis Proteins
  • Qa-SNARE Proteins
  • Vam3 protein, Arabidopsis
  • Green Fluorescent Proteins
  • Acyltransferases
  • TIP1 protein, Arabidopsis
  • RabG3b protein, Arabidopsis
  • rab GTP-Binding Proteins