Role of -675 4G/5G in the plasminogen activator inhibitor-1 gene and -308G/A tumor necrosis factor-α gene polymorphisms in obese Argentinean patients

Genet Test Mol Biomarkers. 2012 May;16(5):372-5. doi: 10.1089/gtmb.2011.0253. Epub 2011 Nov 22.


Aim: Plasminogen activator inhibitor-1 (PAI-1) and tumor necrosis factor-α (TNF-α) are increased in the circulation of obese persons. Because a direct link between PAI-1 and TNF-α in obesity has been observed, they are candidate genes for the development of obesity. We sought to evaluate the relation between the genotypic and allelic frequencies of the -675 4G/5G PAI-1 and -308 G/A TNF-α polymorphisms and their association with the risk for obesity in an Argentinean population.

Methods: A group of 110 consecutive obese persons and a group of 111 lean controls were recruited. Polymerase chain reaction was used to determine the frequency of PAI-1 and TNF-α polymorphisms; serum fasting glucose, insulin, and lipid levels were measured by standard methods. Insulin sensitivity was evaluated by using homeostasis model assessment.

Results: The -308 TNF-α and -675 4G/5G PAI-1 genotype distribution did not significantly differ between the groups (p=0.544 and p=0.327, respectively). Homeostasis model assessment was the only positive independent determinant of body mass index (R(2)=0.493; p<0.001).

Conclusion: The -675 4G/5G PAI-1 and the -308 TNF-α polymorphism variants tested in this study, individually or combined, were not associated with obesity in an Argentinean population.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Argentina
  • Blood Glucose / analysis
  • Body Mass Index
  • Case-Control Studies
  • Female
  • Gene Frequency
  • Genotype
  • Humans
  • Insulin Resistance
  • Male
  • Obesity / genetics*
  • Plasminogen Activator Inhibitor 1 / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length
  • Tumor Necrosis Factor-alpha / genetics*
  • Young Adult


  • Blood Glucose
  • Plasminogen Activator Inhibitor 1
  • Tumor Necrosis Factor-alpha