A liquid phase based C. elegans behavioral analysis system identifies motor activity loss in a nematode Parkinson's disease model

J Neurosci Methods. 2012 Mar 15;204(2):234-7. doi: 10.1016/j.jneumeth.2011.11.015. Epub 2011 Nov 17.

Abstract

Motor activity of Caenorhabditis elegans is widely used to study the mechanisms ranging from basic neuronal functions to human neurodegenerative diseases. It may also serve as a paradigm to screen for potential therapeutic reagents treating these diseases. Here, we developed an automated, 96-well plate and liquid phase based system that quantifies nematode motor activity in real time. Using this system, we identified an adult-onset, ageing-associated motor activity loss in a transgenic nematode line expressing human pathogenic G2019S mutant LRRK2 (leucine-rich repeat kinase 2), the leading genetic cause of Parkinson's disease characterized by dopaminergic neurodegeneration associated motor deficient mainly in elder citizens. Thus, our system may be used as a platform to screen for potential therapeutic drugs treating Parkinson's disease. It can also be used to monitor motor activity of nematodes in liquid phase at similar scenario.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Antiparkinson Agents / therapeutic use
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / genetics
  • Electronic Data Processing / instrumentation
  • Electronic Data Processing / methods
  • Glutamic Acid / genetics
  • Humans
  • Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
  • Levodopa / therapeutic use
  • Motor Activity / genetics
  • Motor Activity / physiology*
  • Mutation / genetics
  • Parkinson Disease / diagnosis
  • Parkinson Disease / drug therapy
  • Parkinson Disease / genetics
  • Parkinson Disease / physiopathology*
  • Protein Serine-Threonine Kinases / genetics*
  • Serine / genetics
  • Software

Substances

  • Antiparkinson Agents
  • Caenorhabditis elegans Proteins
  • Glutamic Acid
  • Serine
  • Levodopa
  • LRRK2 protein, human
  • Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
  • Protein Serine-Threonine Kinases