Abstract
The novel optical system based on converging spherical wave illumination for analysis of bacteria colonies diffraction patterns, is proposed. The complex physical model of light transformation on bacteria colonies in this system, is presented. Fresnel diffraction patterns of bacteria colonies Escherichia coli, Salmonella enteritidis, Staphylococcus aureus grown in various conditions, were examined. It was demonstrated that the proposed system enables the characterization of morphological changes of colony structures basing on the changes of theirs Fresnel diffraction patterns.
© 2011 Optical Society of America
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Absorption / drug effects
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Absorption / radiation effects
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Bacteria / cytology
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Bacteria / drug effects
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Bacteria / growth & development*
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Bacteria / radiation effects*
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Colony Count, Microbial
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Culture Media / pharmacology
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Escherichia coli / cytology
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Escherichia coli / drug effects
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Escherichia coli / growth & development
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Escherichia coli / radiation effects
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Light*
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Optical Devices*
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Salmonella / cytology
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Salmonella / drug effects
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Salmonella / growth & development
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Salmonella / radiation effects
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Spectrum Analysis
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Staphylococcus aureus / cytology
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / growth & development
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Staphylococcus aureus / radiation effects
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Time Factors