Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy

Opt Express. 2011 Nov 7;19(23):23215-26. doi: 10.1364/OE.19.023215.


An investigation is reported of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In particular, two different methods have been developed for in vitro bull sperm head morphometry analysis. We show that semen analysis can be accomplished by means of the proposed techniques . Extraction and measurement of various parameters are performed. It is demonstrated that both proposed methods are efficient to skim the data set in a preselective analysis for discarding anomalous data.

MeSH terms

  • Algorithms
  • Animals
  • Cattle
  • Holography / methods*
  • Image Processing, Computer-Assisted
  • Male
  • Microscopy, Phase-Contrast / methods*
  • Probability
  • Rotation
  • Sperm Head / metabolism*