A continuous and adsorptive bioprocess for efficient production of the natural aroma chemical 2-phenylethanol with yeast

Enzyme Microb Technol. 2011 Apr 7;48(4-5):404-7. doi: 10.1016/j.enzmictec.2011.01.006. Epub 2011 Feb 23.

Abstract

Natural 2-phenylethanol is a high value aroma chemical and can be produced from l-phenylalanine via Ehrlich pathway by yeasts. Due to serious product inhibition, the space-time yield is usually low. A continuous approach using macroporous resin as in situ adsorbent made it possible that the quantity and viability of the cells were improved simultaneously. With Saccharomyces cerevisiae sp. strain R-UV3, the highest space-time yield of 0.90 gL(-1)h(-1) reported so far was obtained.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Bioreactors / microbiology*
  • Biotechnology / methods*
  • Cellulase / metabolism*
  • Cellulose / metabolism
  • Enzyme Activation / drug effects
  • Phenols / isolation & purification
  • Phenols / metabolism
  • Phenols / pharmacology
  • Phenylalanine / metabolism
  • Phenylethyl Alcohol / metabolism*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*

Substances

  • Phenols
  • Phenylalanine
  • Cellulose
  • Cellulase
  • Phenylethyl Alcohol