The effect of strontium incorporation into hydroxyapatites on their physical and biological properties

J Biomed Mater Res B Appl Biomater. 2012 Feb;100(2):562-8. doi: 10.1002/jbm.b.31986. Epub 2011 Nov 24.


In this study, the effects of a series of strontium (Sr)-substituted HA ceramics (0, 1, 5, and 10 mol % Sr substitution) were tested on their physical and biological properties. The crystal structure, composition, and solubility were investigated by TEM, XRD, and solid titration solubility isotherms, respectively. In addition, rat MSCs were cultured with culture media containing ions released from the strontium-substituted HA ceramics as they dissolved. MTT test, alkaline phosphatase activity, and osteoblast transcription factor gene (cbfa1) expression were conducted at different time points. Our results suggested that HA with Sr substitution may change its physical properties, especially its solubility, and consequently enhance undifferentiated MSCs into osteoblast lineage. The results from this and the previous study suggested that 5-10 mol % Sr substitution into HA may be a suitable choice for its use in bone regenerative field.

Keywords: MSCs; differentiation; hydroxyapatite; solubility; strontium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Ceramics* / chemistry
  • Ceramics* / pharmacology
  • Durapatite* / chemistry
  • Durapatite* / pharmacology
  • Materials Testing*
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Osteoblasts / cytology
  • Osteoblasts / metabolism*
  • Rats
  • Strontium* / chemistry
  • Strontium* / pharmacology


  • Durapatite
  • Strontium