Evaluation of wound healing and antimicrobial potentials of Ixora coccinea root extract

Asian Pac J Trop Med. 2011 Dec;4(12):959-63. doi: 10.1016/S1995-7645(11)60226-5.


Objective: To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea (I. coccinea).

Methods: To investigate the wound healing efficacy of root extract of I. coccinea Linn, five groups of animals were divided each containing six animals. Two wound models including incision and excision wound models were used in this study. The parameters studied were tensile strength on incision wound model and in terms of wound contraction for excision wound model were compared with standard Nitrofurazone (NFZ) ointment (0.2% w/w). Six extracts (ethanol, aqueous, petroleum ether, benzene, chloroform and ethyl acetate) of I. coccinea were screened for in vitro growth inhibiting activity against different bacterial strains viz, Staphylococcus aureus, Bacillus pumilius, Enterococcus faecalis, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa and fungi Candida albicans and Aspergillus niger were compared with the standard drugs ciprofloxacin and chloramphenicol for antibacterial and griseofulvin for antifungal screening. The serial dilution and cup (or) well plate methods were used for the antimicrobial study and MIC was determined.

Results: The ethanolic extract showed significant (P<0.001) wound healing activity when compared to standard drug NFZ with respect to normal control group. Amongst all, ethanolic extract showed highly significant antibacterial activity against all bacterial strains used in this study when compared to standard. The aqueous extract showed moderate significant inhibition against all bacterial strains when compared to standard. All the extracts were shown negligible activity against the fungal strains used in this study.

Conclusions: The ethanolic root extract of I. coccinea showed pronounced wound healing and antibacterial activity. The probable reason to heal the wound was that the external application of the extract prevented the microbes to invade through the wound thus the protection of wound occurs against the infection of the various organisms.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Antifungal Agents / pharmacology*
  • Bacterial Infections / drug therapy*
  • Chloramphenicol / pharmacology
  • Ciprofloxacin / pharmacology
  • Disease Models, Animal
  • Griseofulvin / pharmacology
  • Mycoses / drug therapy*
  • Phytotherapy*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plant Roots
  • Rats
  • Rats, Wistar
  • Rubiaceae* / chemistry
  • Tensile Strength / drug effects
  • Wound Healing / drug effects*


  • Anti-Bacterial Agents
  • Antifungal Agents
  • Plant Extracts
  • Griseofulvin
  • Ciprofloxacin
  • Chloramphenicol