Specialized chromosomes and their uses in Caenorhabditis elegans

Methods Cell Biol. 2011;106:23-64. doi: 10.1016/B978-0-12-544172-8.00002-5.


Research on Caenorhabditis elegans involves the use of a wide range of genetic and molecular tools consisting of chromosomal material captured and modified for specific purposes. These "specialized chromosomes" come in many forms ranging from relatively simple gene deletions to complex rearrangements involving endogenous chromosomes as well as transgenic constructs. In this chapter, we describe the specialized chromosomes that are available in C. elegans, their origins, practical considerations, and methods for generation and evaluation. We will summarize their uses for biological studies, and their contribution to our knowledge about chromosome biology.

MeSH terms

  • Alleles
  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans / genetics*
  • Chromosome Aberrations*
  • Chromosome Mapping
  • Chromosomes / genetics*
  • Comparative Genomic Hybridization
  • Genes, Lethal
  • Genetic Engineering / methods*
  • Genetic Markers
  • Genome, Helminth*
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Terminology as Topic


  • Genetic Markers