Eomesodermin controls interleukin-5 production in memory T helper 2 cells through inhibition of activity of the transcription factor GATA3

Immunity. 2011 Nov 23;35(5):733-45. doi: 10.1016/j.immuni.2011.08.017.


The regulation of memory CD4(+) helper T (Th) cell function, such as polarized cytokine production, remains unclear. Here we show that memory T helper 2 (Th2) cells are divided into four subpopulations by CD62L and CXCR3 expression. All four subpopulations produced interleukin-4 (IL-4) and IL-13, whereas only the CD62L(lo)CXCR3(lo) population produced IL-5 accompanied by increased H3-K4 methylation at the Il5 gene locus. The transcription factor Eomesodermin (encoded by Eomes) was highly expressed in memory Th2 cells, whereas its expression was selectively downregulated in the IL-5-producing cells. Il5 expression was enhanced in Eomes-deficient cells, and Eomesodermin was shown to interact with the transcription factor GATA3, preventing GATA3 binding to the Il5 promoter. Memory Th2 cell-dependent airway inflammation was attenuated in the absence of the CD62L(lo)CXCR3(lo) population but was enhanced by Eomes-deficient memory Th2 cells. Thus, IL-5 production in memory Th2 cells is regulated by Eomesodermin via the inhibition of GATA3 activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • GATA3 Transcription Factor / antagonists & inhibitors
  • GATA3 Transcription Factor / metabolism*
  • Gene Expression
  • Immunologic Memory / immunology*
  • Inflammation / immunology
  • Interleukin-5 / biosynthesis*
  • L-Selectin / metabolism
  • Lymphocyte Depletion
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Nude
  • Mice, Transgenic
  • Promoter Regions, Genetic
  • Receptors, CXCR3 / metabolism
  • Respiratory System / immunology
  • T-Box Domain Proteins / genetics
  • T-Box Domain Proteins / metabolism*
  • Th2 Cells / immunology*
  • Th2 Cells / metabolism
  • Transcription, Genetic


  • Eomes protein, mouse
  • GATA3 Transcription Factor
  • Interleukin-5
  • Receptors, CXCR3
  • T-Box Domain Proteins
  • T-box transcription factor TBX21
  • L-Selectin

Associated data

  • GEO/GSE33516