Multimodality functional imaging of spontaneous canine tumors using 64Cu-ATSM and 18FDG PET/CT and dynamic contrast enhanced perfusion CT

Radiother Oncol. 2012 Mar;102(3):424-8. doi: 10.1016/j.radonc.2011.10.021. Epub 2011 Nov 24.


Purpose: To compare the distribution and uptake of the hypoxia tracer (64)Cu-diacetyl-bis(N(4)-methylthiosemicarbazone) ((64)Cu-ATSM) PET/CT, FDG PET/CT and dynamic contrast enhanced perfusion CT (DCE-pCT) in spontaneous canine tumors. In addition (64)Cu-ATSM distribution over time was evaluated.

Methods and materials: Nine spontaneous cancer-bearing dogs were prospectively enrolled. FDG (1h pi.) and (64)Cu-ATSM (3 and 24h pi.) PET/CT were performed over three consecutive days. DCE-pCT was performed on day 2. Tumor uptake of FDG and (64)Cu-ATSM was assessed semi-quantitatively and the distribution of FDG, (64)Cu-ATSM and CT perfusion parameters correlated.

Results: (64)Cu-ATSM distribution on scans performed 24h apart displayed moderate to strong correlation; however, temporal changes were observed. The spatial distribution pattern of (64)Cu-ATSM between scans was moderately to strongly positively correlated to FDG, whereas the correlation of CT perfusion parameters to FDG and to (64)Cu-ATSM yielded more varying results.

Conclusions: (64)Cu-ATSM uptake was positively correlated to FDG. (64)Cu-ATSM was found to be relatively stable between PET scans performed at different time points, important temporal changes were however observed in hypo-perfused regions. These findings potentially indicate that prolonged uptake periods for (64)Cu-ATSM imaging may be needed. Although a moderate to strong correlation between (64)Cu-ATSM and FDG PET/CT is observed, the two tracers provide different biological information with an overlapping spatial distribution.

MeSH terms

  • Animals
  • Contrast Media
  • Copper Radioisotopes*
  • Dog Diseases / diagnostic imaging*
  • Dogs
  • Fluorodeoxyglucose F18*
  • Multimodal Imaging / methods*
  • Neoplasms / diagnostic imaging
  • Neoplasms / veterinary*
  • Organometallic Compounds*
  • Perfusion Imaging / methods*
  • Positron-Emission Tomography*
  • Radiographic Image Enhancement / methods*
  • Radiopharmaceuticals*
  • Thiosemicarbazones*
  • Tomography, X-Ray Computed*


  • Contrast Media
  • Copper Radioisotopes
  • Organometallic Compounds
  • Radiopharmaceuticals
  • Thiosemicarbazones
  • copper (II) diacetyl-di(N(4)-methylthiosemicarbazone)
  • Fluorodeoxyglucose F18