Chemical analysis of an immunostimulating (1→4)-, (1→6)-branched glucan from an edible mushroom, Calocybe indica

Eshita Kar Mandal; Kousik Maity; Saikat Maity; Sanjoy K Gantait; Birendra Behera; Tapas K Maiti; Samir R Sikdar; Syed S Islam

doi:10.1016/j.carres.2011.10.040

Chemical analysis of an immunostimulating (1→4)-, (1→6)-branched glucan from an edible mushroom, Calocybe indica

Carbohydr Res. 2012 Jan 10;347(1):172-7. doi: 10.1016/j.carres.2011.10.040. Epub 2011 Nov 4.

Authors

Eshita Kar Mandal¹, Kousik Maity, Saikat Maity, Sanjoy K Gantait, Birendra Behera, Tapas K Maiti, Samir R Sikdar, Syed S Islam

Affiliation

¹ Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.

PMID: 22119439
DOI: 10.1016/j.carres.2011.10.040

Abstract

An immunostimulating water-soluble glucan was isolated from hot aqueous extract of fruit bodies of an edible mushroom Calocybe indica. Structural investigation of the glucan was carried out using acid hydrolysis, methylation analysis, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as [see figure in text]. This glucan stimulated the splenocytes and thymocytes.

MeSH terms

Adjuvants, Immunologic / chemistry*
Adjuvants, Immunologic / isolation & purification
Adjuvants, Immunologic / pharmacology*
Agaricales / chemistry*
Animals
Carbohydrate Sequence
Cell Proliferation / drug effects
Glucans / chemistry*
Glucans / isolation & purification
Glucans / pharmacology*
Mice
Molecular Sequence Data
Spleen / cytology
Thymocytes / cytology
Thymocytes / drug effects

Substances

Adjuvants, Immunologic
Glucans