The hepatoprotective potential of Spirulina and vitamin C supplemention in cisplatin toxicity

Food Funct. 2012 Feb;3(2):164-9. doi: 10.1039/c1fo10172b. Epub 2011 Nov 28.


Spirulina platensis is a microalgae with potent dietary phyto-antioxidant, anti-inflammatory and anti-carcinogenic properties. We investigated the mechanism of cisplatin induced hepatotoxicity and whether this natural antioxidant provided protection against cisplatin hepatotoxicity. The study was carried out in a mice model where the animals were segregated into different groups according to their treatments, e.g. control group with no treatment, cisplatin treated, cisplatin + Spirulina treated, cisplatin + vitamin C treated and cisplatin + Spirulina + vitamin C treated. The liver marker enzymes were found to be elevated following cisplatin treatment, signifying hepatotoxicity. The supplementation of Spirulina and vitamin C could effectively bring down the levels of these enzymes. Light microscopy also showed that cisplatin treatment induced liver injury and that histopathological abnormalities were prevented by Spirulina and vitamin C supplementation. This protective effect was further substantiated by the estimation of antioxidant levels and extent of lipid peroxidation in the Spirulina, vitamin C and Spirulina + vitamin C supplemented groups as compared to cisplatin alone.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Ascorbic Acid / administration & dosage*
  • Aspartate Aminotransferases / metabolism
  • Cisplatin / toxicity*
  • Diet
  • Dietary Supplements*
  • Female
  • L-Lactate Dehydrogenase / metabolism
  • Lipid Peroxidation / drug effects
  • Liver / drug effects*
  • Liver / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Spirulina / chemistry*
  • Vitamins / administration & dosage*


  • Vitamins
  • L-Lactate Dehydrogenase
  • Aspartate Aminotransferases
  • Alkaline Phosphatase
  • Ascorbic Acid
  • Cisplatin