Bioassay of interleukin-1 in serum and plasma following removal of inhibitory activity with polyethylene glycol

J Immunol Methods. 1990 Oct 4;133(1):127-31. doi: 10.1016/0022-1759(90)90326-q.

Abstract

Serum and plasma normally inhibit the responsiveness of the indicator cells used in bioassays for interleukin-1 (IL-1). A precipitation step with 12% polyethylene glycol (PEG) was found to remove material responsible for such inhibition. The IL-1 bioassay could be performed in the presence of a 3% concentration of PEG and recovery of added natural IL-1 from plasma or serum was essentially complete.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Assay / methods
  • Blood Proteins / drug effects
  • Humans
  • Interleukin-1 / antagonists & inhibitors
  • Interleukin-1 / blood*
  • Polyethylene Glycols / pharmacology*
  • Rats
  • Recombinant Proteins
  • Tumor Cells, Cultured

Substances

  • Blood Proteins
  • Interleukin-1
  • Recombinant Proteins
  • Polyethylene Glycols