Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells

Methods Mol Biol. 2012;815:253-63. doi: 10.1007/978-1-61779-424-7_19.

Abstract

In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein-protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Fluorescence Resonance Energy Transfer / methods*
  • Humans
  • Luminescent Measurements / methods*
  • Luminescent Proteins / metabolism
  • Protein Binding
  • Protein Interaction Mapping / methods*
  • Recombinant Fusion Proteins / metabolism
  • Transfection / methods

Substances

  • Luminescent Proteins
  • Recombinant Fusion Proteins