Metabolite analysis of Cannabis sativa L. by NMR spectroscopy

Methods Mol Biol. 2012:815:363-75. doi: 10.1007/978-1-61779-424-7_27.

Abstract

NMR-based metabolomics is an analytical platform, which has been used to classify and analyze Cannabis sativa L. cell suspension cultures and plants. Diverse groups of primary and secondary metabolites were identified by comparing NMR data with reference compounds and/or by structure elucidation using ¹H-NMR, J-resolved, ¹H-¹H COSY, and ¹H-¹³C HMBC spectroscopy. The direct extraction and the extraction by indirect fractionation are two suitable methods for the C. sativa sample preparation. Quantitative analyses could be performed without requiring fractionation or isolation procedures.

MeSH terms

  • Cannabis / metabolism*
  • Cell Culture Techniques
  • Chemical Fractionation
  • Flowers / metabolism*
  • Magnetic Resonance Spectroscopy
  • Metabolome*
  • Multivariate Analysis
  • Plant Extracts / isolation & purification
  • Plant Leaves / metabolism*
  • Principal Component Analysis

Substances

  • Plant Extracts