The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2O2 induction of the NF-kappaB pathway through inhibition of Ikappaalpha phosphorylation in MCF7 breast cancer cells

Int J Med Mushrooms. 2011;13(1):19-25. doi: 10.1615/intjmedmushr.v13.i1.30.

Abstract

Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-kappaB) transcription factor. The IC50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 microg/mL, and the IC50 value of C. comatus ethyl acetate extract was only 32 microg/ mL. Our results also showed that both extracts significantly affected IkappaBalpha phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-kappaB pathway inhibitor, and seemed to be the most active inhibitor of H2O2-dependent IkappaBalpha phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-kappaB function and also possibly acts as an antitumor agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / chemistry
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / metabolism
  • Cell Line, Tumor
  • Cell Survival
  • Complex Mixtures / isolation & purification
  • Complex Mixtures / pharmacology*
  • Coprinus / chemistry*
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • I-kappa B Kinase / antagonists & inhibitors
  • I-kappa B Kinase / drug effects*
  • I-kappa B Kinase / metabolism
  • I-kappa B Proteins / drug effects*
  • I-kappa B Proteins / metabolism
  • Inhibitory Concentration 50
  • NF-KappaB Inhibitor alpha
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / drug effects*
  • NF-kappa B / metabolism
  • Phosphorylation / drug effects
  • Signal Transduction / drug effects
  • Time Factors

Substances

  • Acetates
  • Complex Mixtures
  • I-kappa B Proteins
  • NF-kappa B
  • NFKBIA protein, human
  • NF-KappaB Inhibitor alpha
  • ethyl acetate
  • Hydrogen Peroxide
  • I-kappa B Kinase