Trials for the control of trichodinosis and gyrodactylosis in hatchery reared Oreochromis niloticus fries by using garlic

Vet Parasitol. 2012 Apr 30;185(2-4):57-63. doi: 10.1016/j.vetpar.2011.10.035. Epub 2011 Nov 6.

Abstract

The present work was designed to study the prevalence of trichodinosis and gyrodactylosis in Oreochromis niloticus fries, and to test the therapeutic efficacy and preventive efficacy of garlic oil and crushed garlic cloves. Trichodinosis and gyrodactylosis are ectoparasitic diseases that affect most warm freshwater fish, especially fries and fingerlings. In a private O. niloticus fish hatchery, the prevalence of trichodinosis in 5-, 15- and 30-day-old-fries was 37%, 23% and 40.5%, respectively. The highest infection intensity was detected in 30-day-old-fries. The gyrodactylosis was reported only in combination with trichodinosis. In addition, we found that its prevalence in 5-, 15- and 30-day-old-fries was 17%, 19.5% and 29%, respectively. Mortality rate of fry in the first month of life was 53% as a result of injury to these two types of parasites. The garlic oil and crushed garlic cloves were tested in both in vitro and earthen ponds of the hatchery. Using 2-, 2.5- and 3-ppt (parts per thousand) garlic oil for 4h in vitro water bath treatment resulted in 100% recovery, while 1 and 1.5 ppt garlic oil, respectively, needed 24 and 16 h to treat the infected fries. The treatment by 3 ppt garlic oil as a water bath for 1h treated the two diseases in 55% in 7 days from application in the hatchery earthen pond. In the mean time, 300 mg L(-1) crushed garlic cloves as an indefinite bath in the hatchery earthen pond eliminated 68% of the diseases. The same protocol for preventing the two diseases resulted in obtaining 65% and 75% of parasite free fries, for garlic oil and crushed garlic cloves, respectively, compared to 53% of the control fries.

Publication types

  • Controlled Clinical Trial

MeSH terms

  • Allyl Compounds / pharmacology*
  • Animals
  • Antiparasitic Agents / therapeutic use
  • Aquaculture
  • Cichlids*
  • Ciliophora / drug effects
  • Ciliophora Infections / drug therapy
  • Ciliophora Infections / parasitology
  • Ciliophora Infections / veterinary*
  • Fish Diseases / parasitology
  • Fish Diseases / prevention & control*
  • Garlic*
  • Platyhelminths / drug effects
  • Sulfides / pharmacology*
  • Trematode Infections / drug therapy
  • Trematode Infections / parasitology
  • Trematode Infections / veterinary*

Substances

  • Allyl Compounds
  • Antiparasitic Agents
  • Sulfides
  • allyl sulfide