Reciprocal regulation between AtNRT2.1 and AtAMT1.1 expression and the kinetics of NH(4)(+) and NO(3)(-) influxes

J Plant Physiol. 2012 Feb 15;169(3):268-74. doi: 10.1016/j.jplph.2011.10.011. Epub 2011 Dec 3.


Our results show that AtNRT2.1 expression has a positive effect on the NH(4)(+) ion influx, mediated by the HATS, as also occurs with AtAMT1.1 expression on the NO(3)(-) ion influx. AtNRT2.1 expression plays a key role in the regulation of AtAMT1.1 expression and in the NH(4)(+) ion influx, differentiating the nitrogen source, and particularly, the lack of it. Nitrogen starvation produces a compensatory effect by AtAMT1.1 when there is an absence of the AtNRT2.1 gene. Our results also show that, in the atnrt2 mutant lacking both AtNRT2.1 and AtNRT2.2, gene functions present different kinetic parameters on the NH(4)(+) ion influx mediated by the HATS, according to the source and availability of nitrogen. Finally, the absence of AMT1.1 also produces changes in the kinetic parameters of the NO(3)(-) influx, showing different V(max) values depending on the source of nitrogen available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anion Transport Proteins / biosynthesis*
  • Anion Transport Proteins / genetics
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / biosynthesis*
  • Arabidopsis Proteins / genetics
  • Cation Transport Proteins / biosynthesis*
  • Cation Transport Proteins / genetics
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Genetic Variation
  • Genotype
  • Ion Transport
  • Nitrates / metabolism*
  • Nitrogen / deficiency
  • Nitrogen / metabolism
  • Plant Proteins / biosynthesis*
  • Plant Proteins / genetics
  • Quaternary Ammonium Compounds / metabolism*


  • Anion Transport Proteins
  • Arabidopsis Proteins
  • AtNRT2.1 protein, Arabidopsis
  • Cation Transport Proteins
  • Nitrates
  • Plant Proteins
  • Quaternary Ammonium Compounds
  • ammonium transporters, plant
  • Nitrogen